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ec-specific culture medium  (Lonza)


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    Structured Review

    Lonza ec-specific culture medium
    Ec Specific Culture Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ec-specific+medium/ec+specific+culture+medium/pmc11535355-50-21-24
    Average 90 stars, based on 1 article reviews
    ec-specific culture medium - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo
    Article Snippet: hASMCs (Lonza, Inc., Allendale, NJ, USA) were cultured in an SMC-specific medium (SmGM-2, Smooth Muscle Growth Medium 2, Lonza) and harvested between passages 4 and 8 for all seeding experiments. hAECs (Lonza) were cultured in an EC-specific medium (EGM-2, Endothelial Growth Medium 2, Lonza) and harvested between passages 6 and 8.

    Article Title: Defective p27 phosphorylation at serine 10 affects vascular reactivity and increases abdominal aortic aneurysm development via Cox-2 activation.
    Article Snippet: The rings were incubated for 1 week in EC-specific medium (DMEM:F12, Lonza) containing 1% penicillin/streptomycin, 0.4 mM L-glutamine, 10 mM Hepes, 1 μg/ml fungizone, 10% fetal bovine serum (FBS), 0.1 mg/ml heparin (SigmaAldrich), and 50 μg/ml EC growth supplement (Becton Dickinson). mAECs that had migrated from the rings onto the plate were selected after incubation with CD102 antibody (Purified Rat Anti-Mouse CD102 - ICAM-2 Monoclonal Antibody, BD Pharmigen) followed by incubation with a secondary antibody linked to magnetic beads (Dynabeads Sheep anti-Rat IgG, Invitrogen).

    Article Title: Rock2 heterozygosity improves recognition memory and endothelial function in a mouse model of 16p11.2 deletion autism syndrome.
    Article Snippet: For a pure EC population, EC-specific medium (Lonza, CC-3202) was replaced every 48 hrs until cells were at least 90 % confluent [14].

    Purification:

    Article Title: Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo
    Article Snippet: hASMCs (Lonza, Inc., Allendale, NJ, USA) were cultured in an SMC-specific medium (SmGM-2, Smooth Muscle Growth Medium 2, Lonza) and harvested between passages 4 and 8 for all seeding experiments. hAECs (Lonza) were cultured in an EC-specific medium (EGM-2, Endothelial Growth Medium 2, Lonza) and harvested between passages 6 and 8.

    Article Title: Defective p27 phosphorylation at serine 10 affects vascular reactivity and increases abdominal aortic aneurysm development via Cox-2 activation.
    Article Snippet: The rings were incubated for 1 week in EC-specific medium (DMEM:F12, Lonza) containing 1% penicillin/streptomycin, 0.4 mM L-glutamine, 10 mM Hepes, 1 μg/ml fungizone, 10% fetal bovine serum (FBS), 0.1 mg/ml heparin (SigmaAldrich), and 50 μg/ml EC growth supplement (Becton Dickinson). mAECs that had migrated from the rings onto the plate were selected after incubation with CD102 antibody (Purified Rat Anti-Mouse CD102 - ICAM-2 Monoclonal Antibody, BD Pharmigen) followed by incubation with a secondary antibody linked to magnetic beads (Dynabeads Sheep anti-Rat IgG, Invitrogen).

    Article Title: Rock2 heterozygosity improves recognition memory and endothelial function in a mouse model of 16p11.2 deletion autism syndrome.
    Article Snippet: For a pure EC population, EC-specific medium (Lonza, CC-3202) was replaced every 48 hrs until cells were at least 90 % confluent [14].

    Magnetic Beads:

    Article Title: Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo
    Article Snippet: hASMCs (Lonza, Inc., Allendale, NJ, USA) were cultured in an SMC-specific medium (SmGM-2, Smooth Muscle Growth Medium 2, Lonza) and harvested between passages 4 and 8 for all seeding experiments. hAECs (Lonza) were cultured in an EC-specific medium (EGM-2, Endothelial Growth Medium 2, Lonza) and harvested between passages 6 and 8.

    Article Title: Defective p27 phosphorylation at serine 10 affects vascular reactivity and increases abdominal aortic aneurysm development via Cox-2 activation.
    Article Snippet: The rings were incubated for 1 week in EC-specific medium (DMEM:F12, Lonza) containing 1% penicillin/streptomycin, 0.4 mM L-glutamine, 10 mM Hepes, 1 μg/ml fungizone, 10% fetal bovine serum (FBS), 0.1 mg/ml heparin (SigmaAldrich), and 50 μg/ml EC growth supplement (Becton Dickinson). mAECs that had migrated from the rings onto the plate were selected after incubation with CD102 antibody (Purified Rat Anti-Mouse CD102 - ICAM-2 Monoclonal Antibody, BD Pharmigen) followed by incubation with a secondary antibody linked to magnetic beads (Dynabeads Sheep anti-Rat IgG, Invitrogen).

    Article Title: Rock2 heterozygosity improves recognition memory and endothelial function in a mouse model of 16p11.2 deletion autism syndrome.
    Article Snippet: For a pure EC population, EC-specific medium (Lonza, CC-3202) was replaced every 48 hrs until cells were at least 90 % confluent [14].



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